Effect of osterix and osteocalcin enhancement by quercetin (3,3′,4′,5,7-pentahydroxyflavone) on osteoblast hfob 1.19 cell line

Aditama, Agnis Pondineka Ria, Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X and Muslikh, Faisal Akhmal (2022) Effect of osterix and osteocalcin enhancement by quercetin (3,3′,4′,5,7-pentahydroxyflavone) on osteoblast hfob 1.19 cell line. International Journal of Applied Pharmaceutics, 14. pp. 32-35. ISSN 0975-7058

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Abstract

Objective: This study was aimed at investigating the effect of quercetin (3,3′,4′,5,7-pentahydroxyflavone) as a phytoestrogen in the treatment of estrogen deficiency-induced osteoporosis, through the measurement of osterix and osteocalcin expressions on osteoblast hFOB 1.19 cell line.

Methods: hFOB 1.19 cells were cultured in 24-well microplates, induced with 10 ng/ml TNF-α and incubated for 24 h. TNF-α induction was used to create an estrogen deficiency condition. Quercetin was then added at 10 µM concentration. The immunocytochemistry double staining method was performed with anti-rabbit osterix primary antibody and anti-mouse osteocalcin primary antibody. The cells were then incubated at 4 °C overnight. Finally, an anti-rabbit secondary antibody FITC and anti-mouse secondary antibody rhodamine were added before the cells were analyzed using a Confocal Instrument Laser Scanning Microscopy (CLSM) at 488 and 543 nm.

Results: Quercetin increased the expressions of both osterix and osteocalcin in the osteoblast hFOB 1.19 cell line compared to the negative controls (p<0.005), with expression values of 57852*±3878.71 AU and 24161.75*±1498.65 AU, respectively.

Conclusion: Quercetin shows an anti-osteoporosis effect by increasing the expressions of both osterix and osteocalcin in osteoblast hFOB 1.19 cell line.

Item Type:	Journal Article
Keywords:	quercetin; anti-osteoporosis; osteoblast; hfob 1.19 cell line; osterix; osteocalcin
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Burhan Ma'arif
Date Deposited:	16 Feb 2022 14:13

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