Metabolite profiling and activity study of ethanol extract of Chrysophyllum cainito L. leaves in increasing bone density in male mice

Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X, Putra, Kurniawan H. P., Arifin, Miftah S., Rukiana, Rukiana, Amiruddin, Reyhan, Bhagawan, Weka Sidha, Suryadinata, Arief, Inayatilah, Fidia Rizkiah, Mutiah, Roihatul and Aditama, Agnis P. (2022) Metabolite profiling and activity study of ethanol extract of Chrysophyllum cainito L. leaves in increasing bone density in male mice. Thai Journal of Pharmaceutical Sciences, 46 (1). pp. 69-74. ISSN 1905-4637

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Abstract

Phytoestrogen is a group compound which has an estrogen-like structure or function. One of its roles was in the bone formation. Chrysophyllum cainito L. is a plant that contains phytoestrogen. The purpose of this research is to determine the metabolite profile and activity of ethanol extract of C. cainito leaves to increase trabecular femur bone density in male mice. The metabolite profiling analysis was done using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry and analyzed using Masslynx 4.1 software. Four doses of ethanol extract of C. cainito leaves were prepared for treatment. Which are 2,14,48, and 316 mg/ body weight (BW) mice/day in 4 weeks, after the mice were induced by dexamethasone 0.0029 mg/ BW mice/day as osteoporosis model, and induction of alendronate 0.026 mg/BW mice/day as a positive control. The metabolite profiling showed a total of 41 compounds, with some compounds have activity as estrogenic such as myricetin and dibutyl phthalate. The activity test showed that ethanol extract of C. cainito leaves in all doses can increase the trabecular femur bone densityin male mice, with an optimum dose of 16 mg/BW mice/day. This activity is probably due to myricetin and dibutyl phthalate that act as phytoestrogens in C. cainito, that can replace the function of estrogen in its bond with bone-estrogen receptor.

Item Type:	Journal Article
Keywords:	Metabolite profiling; Chrysophyllum cainito L.; bone density; phytoestrogen
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Burhan Ma'arif
Date Deposited:	08 Mar 2022 14:03

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