The effect of ethyl acetate fraction of Marsilea crenata presl. leaves in increasing osterix expression in hFOB 1.19 cells

Aditama, Agnis P., Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X, Laswati, Hening and Agil, Mangestuti (2022) The effect of ethyl acetate fraction of Marsilea crenata presl. leaves in increasing osterix expression in hFOB 1.19 cells. International Journal of Health Sciences, 6 (2). pp. 789-796. ISSN 2550-696X

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Abstract

Marsilea crenata Presl. leaves contain phytoestrogens that are suspected to play a role in increasing bone formation. Bone formation activity is defined by the expression of Osterix, a transcription factor that plays a key role in bone development. This study aims to prove that the ethyl acetate fraction of Marsilea crenata Presl. leaves can enhance the bone formation process in hFOB 1.19 cellsin a TNF-α dependent manner, by Osterix measurement. The hFOB 1.19 cells were grown in 24-well microplates and then treated with 10 ng/ml TNF-α for 24 hours. The ethyl acetate fraction of Marsilea crenata Presl. leaves were then added in concentrations of 62.5, 125, and 250 ppm. Genistein as positive control was added at a concentration of 2.5 g/L. The expression of Osterix was then examined using an immunocytochemistry technique with CLSM to assess bone-forming activity. The results show that ethyl acetate fraction Marsilea crenata Presl. leaves can boost Osterix expression in hFOB 1.19 cells, with an optimal concentration was 250 ppm at p<0.005. The ethyl acetate fraction of Marsilea crenata Presl. leaves can increase Osterix in osteoblast hFOB 1.19 cell, indicating improved bone-forming activity.

Item Type:	Journal Article
Keywords:	bone formation; ethyl acetate fraction; Hfob 1.19; marsilea crenata presl; osterix
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Burhan Ma'arif
Date Deposited:	06 Jun 2022 14:38

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