Runt-related transcription factor 2 (Runx2) measurement in phytoestrogen-induced bone: a comparison of western blot and immunohistochemistry methods

Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X, Sholihah, Fariza Amanatul, Mahardiani, Anisah, Fauziyah, Begum, Mirza, Denis Mery and Agil, Mangestuti (2022) Runt-related transcription factor 2 (Runx2) measurement in phytoestrogen-induced bone: a comparison of western blot and immunohistochemistry methods. Biomedical and Pharmacology Journal, 15 (2). pp. 1039-1052. ISSN 2456–2610

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Abstract

Estrogen deficiency can contribute to osteoporosis in postmenopausal women. Phytoestrogens are becoming more widely recognized as potential estrogen replacement therapy. The administration of phytoestrogens can cause bone formation, which is marked by an increase in Runx2 expression in osteoblast cells and can be seen using western blot and immunohistochemistry approaches. This review aimed to compare the detection methods of Runx2 in phytoestrogen-induced bone tissue using western blots and immunohistochemistry. Selectivity, sensitivity, processing time, and cost-effectiveness were the parameters that were compared. This review was done by identifying articles in several databases (Google Scholar, PubMed, and Science Direct). The process of selecting the articles used the PRISMA guidelines to create a flowchart with inclusion and exclusion study criteria. Meta-synthesis was done to analyze, identify, and interpret all of the data in the articles systematically. 70 articles in total were obtained from the selection process, with 21 articles being relevant to the topic. The result shows that the selectivity and sensitivity of western blot for detecting Runx2 on tissue were 93.5–100%, respectively, whereas immunohistochemistry selectivity and sensitivity were 45–99.5%, respectively. Compared to immunohistochemistry, western blot can save up to 57.26%. Immunohistochemistry takes 46 hours to process, while Western blot takes 25 hours and 20 minutes. In comparison to immunohistochemistry, the western blot is more selective, sensitive, rapid and affordable for detecting Runx2 in bone tissue.

Item Type:	Journal Article
Keywords:	immunohistochemistry; phytoestrogens; runx2; western blot
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Burhan Ma'arif
Date Deposited:	20 Jul 2022 11:24

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