The combination of decaffeinated coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7

Saifur, Rohman Mohammad, Widya, Sishartami Lintang, Nashi, Widodo, Rachmawati, Ermin ORCID: https://orcid.org/0000-0003-1045-7066 and Mifetika, Lukitasari (2022) The combination of decaffeinated coffee (Coffea canephora) and Green Tea (Camellia sinensis) Reduced PSGL-1 Glycosylation by GALNT2 in Ox-LDL-induced RAW 264.7. International Journal of Nutrition, Pharmacology, Neurological Diseases, 12 (3). pp. 112-119. ISSN 2231-0738

Text 11719.pdf - Published Version Restricted to Repository staff only Download (1MB)

Abstract

Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 mg/mL + green tea 80 mg/mL; coffee 160 mg/mL + green tea 160 mg/mL; coffee 320 mg/mL + green tea 320 mg/mL), respectively. Then, cells stimulated with 8 mg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells

Item Type:	Journal Article
Keywords:	coffee; green tea; GALNT2; PSGL-1; oxidized-LDL
Divisions:	?? JDok ??
Depositing User:	dr Ermin rachmawati
Date Deposited:	24 Oct 2022 09:47

Downloads

Origin of downloads

Actions (login required)

View Item