Phenotypic variation and RAPD Polymorphism of Pisang Kepok local cultivars (Musa acuminata x Musa balbisiana, ABB, Saba Subgroup)

Wahyudi, Didik, Ilmi, Zahrbatul Lil and Hapsari, Lia (2022) Phenotypic variation and RAPD Polymorphism of Pisang Kepok local cultivars (Musa acuminata x Musa balbisiana, ABB, Saba Subgroup). Biotropika Journal of Tropical Biology, 10 (3). pp. 176-184. ISSN 2302-7282

Preview

Text 12100.pdf - Published Version Available under License Creative Commons Attribution Share Alike. Download (671kB) | Preview

Abstract

Pisang Kepok is a major local banana cultivar in Indonesia with high social, economy and cultural value. Particularly on the island of Java, there are several variations of Pisang Kepok recognized with their own local names, which makes difficulties in taxonomic identification and grouping. Morphological features are still used in conventional banana cultivar classification studies, but they are deemed less precise due to their subjectivity, thus it supposed to be complemented with molecular characterization. This study aims to identify the phenotypic variation of Pisang Kepok local cultivars, also their genetic polymorphism using Random Amplified Polymorphic DNA marker. RAPD primers used include OPA 2, OPA 3, OPA 4, OPA 11, OPA 12 and OPA 18. Clustering analysis both phenotypic and genetic were performed using Paleontological Statistics (PAST) pogram version 4.02. The morphological characterization identified 4 variants of Pisang Kepok (Kepok Abang, Kepok Putih, Kepok Manurun, and Kepok Australi), which confirmed included as ABB genome group. Phenotypic characterization resulted that Pisang Kepok cultivars were separated into 3 clusters based on their local name, with a high similarity value of >90%. PCA biplot showed that the fruit flesh color was considered as the most important character contributed to the variation of Pisang Kepok cultivars. RAPD marker also showed that each specimen was grouped according to its local name. Both of morphology and molecular (RAPD) are showed that Pisang Kepok has closer branches with Pisang Klutuk than with Pisang Barlin.

Item Type:	Journal Article
Keywords:	characterization; molecular; morphology; Pisang Kepok; RAPD
Subjects:	06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology
Depositing User:	Didik Wahyudi
Date Deposited:	16 Nov 2022 14:15

Downloads

Origin of downloads

Actions (login required)

View Item