Role of ISSR molecular marker in analysis of genetic diversity of mutant glycine soja through mutation induction with combination of gamma ray irradiation and EMS (Ethyl methane sulfonate)

Savitri, Evika Sandi ORCID: https://orcid.org/0000-0002-2068-0563 and Fauziah, Shaddiqah Munawaroh (2020) Role of ISSR molecular marker in analysis of genetic diversity of mutant glycine soja through mutation induction with combination of gamma ray irradiation and EMS (Ethyl methane sulfonate). Journal of Applied Biological Sciences, 14 (1). pp. 113-123. ISSN 2146-0108

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Abstract

Mutation induction in soybeans is performed to achieve a high degree of genetic diversity as a basis for plant breeding in an attempt to obtain desired varieties. It is performed physically using gamma rays and chemically using EMS mutagen. This research was intended to figure out the effectiveness of the combination of chemical (EMS) and physical mutagens (gamma rays) in the induction of genetic diversity in black soybeans. Mutation detection can be carried out with molecular markers to characterize the genetic diversity of a crop. The mutation induction method employed in this research involved EMS and gamma-ray treatments. This research made use of 5-primer ISSR molecular marker. The UBC 810, UBC 811, UBC 812, UBC 828, and K18 primers used had successfully amplified and demonstrated genetic diversity in the black soybeans which were subjected to gamma-ray irradiation and EMS administration. The study results indicate that gamma-ray irradiation treatment yielded a higher extent of polymorphism than did control and EMS treatment, thus fit to be applied to candidate parent plants for further treatments.

Item Type:	Journal Article
Keywords:	mutation induction, gamma rays, EMS, ISSR, black soybeans
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Sandi Savitri
Date Deposited:	13 Apr 2023 08:47

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