Chrysanthemum cinerariifolium (Trev.) Vis leaves extract reduces the level of macrophages, interleukin-17, and keratinization in oral squamous cell carcinoma rat model

Listiyana, Anik ORCID: https://orcid.org/0000-0001-6476-2977, Kristanti, Risma Aprinda, Dharmayanti, Nadya, Hidayatulloh, Rizkia Milladina and Baraja, Sakinah (2023) Chrysanthemum cinerariifolium (Trev.) Vis leaves extract reduces the level of macrophages, interleukin-17, and keratinization in oral squamous cell carcinoma rat model. Research Journal of Pharmacy and Technology, 16 (3). pp. 1180-1186. ISSN 0974-360X

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Abstract

Chrysanthemum cinerariifolium (Trev.) Vis. leaves have potential anticancer activity and are expected to inhibit oral squamous cell carcinoma (OSCC) progression. The present study aimed to determine the effect of ethanol extract of C. cinerariifolium leaves on the number of macrophages, IL-17 levels, and degree of keratinization in the OSCC rat model. Thirty male Sprague Dawley rats (weighing 80-160g, 2-3 months old) were randomly divided into five groups (n=6): (1) negative control (healthy rat, untreated); (2) positive control (OSCC rat); (3-5) dose 1, 2 and 3 (OSCC rat received 50, 100 and 200mg/kg BW of C. cinerariifolium leaves, respectively). The tongues of the rats were prepared for Hematoxylin-Eosin (HE) staining and immunohistochemistry. The number of macrophages was determined under a light microscope with 400x magnification. The level of IL-17 was examined using an ELISA assay. The degree of keratinization was calculated using the ImageJ software. All doses of C. cinerariifolium leaves extract could reduce the number of macrophages in OSCC rats. The levels of IL-17 in OSCC rats were decreased after being treated with 100 and 200mg/kg BW of C. cinerariifolium leaves extract. The high dose of C. cinerariifolium leaves extract (200mg/kg BW) reduced the degree of keratinization in the OSCC rat model. The study revealed that C. cinerariifolium leaves extract could inhibit the progression of OSCC by reducing macrophage, IL-17 and keratinization.

Item Type:	Journal Article
Keywords:	CD68; Chrysanthemum cinerariifolium; DMBA; IL-17; OSCC; keratinization
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry > 110505 Oral Medicine and Pathology 11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111299 Oncology and Carcinogenesis not elsewhere classified 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified 11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User:	Anik Listiyana
Date Deposited:	27 Apr 2023 19:42

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