Dimensions
DimensionsAnggraini, Wirda, Purwanto, Djoko Agus, Kusumawati, Idha, Isnaeni, Isnaeni and Suryanto, Suryanto (2023) Influence of the environment on biofilm formation Candida albicans of vulvovaginal candidiasis isolate patient. Pharmacognosy Journal, 5 (1). pp. 216-222. ISSN 09753575
|
Text
14517.pdf - Published Version Available under License Creative Commons Attribution. Download (324kB) | Preview |
Abstract
Context: Candida albicans is a type of fungus that can produce biofilms and may cause Vulvovaginal Candidiasis (VVC) disease. We investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Biofilm formation is influenced by several factors such as environments and nutrients. Objectives: To investigated the effect of environment on biofilm formation of C. albicans patient isolates and ATCC 14053. Methods: The samples using C. albicans ATCC 14053, C. albicans, which may form biofilms, was isolated from patient Dermatology and Venereology and Obstetrics and Gynecology from a hospital in Malang. TCP (A tissue Culture Plate) is the biofilm formation method used. Results: Biofilm formation took 48-72 hours at 25 °C and 96-120 hours at 37 °C. Based on the result biofilm formation of C. albicans is influenced by environmental factors and characterized by a high OD value. Conclusions: Biofilm formation is accelerated in temperature incubation needed at 25 °C for 48-72 hours, using biomass 107 CFU/mL, nutrition using Potato Dextrose Broth media and 1% glucose, and the solvent of 30% acetic acid to obtain acid condition.
| Item Type: | Journal Article |
|---|---|
| Keywords: | biofilm formation; Candida albicans; sabourau dextrose broth; potato dextrose broth; vulvovaginal candidiasis |
| Subjects: | 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology |
| Divisions: | Faculty of Medical and Health Sciences > Department of Pharmacy |
| Depositing User: | Wirda Anggraini |
| Date Deposited: | 15 May 2023 13:40 |
Downloads
Downloads per month over past year
Origin of downloads
Actions (login required)
 |
View Item |
