Responsive Banner

Metabolite profiling of compounds from Sargassum polycystum using UPLC-QToF-MS/MS

Riwanti, Pramudita, Prasetyanti, Intan Kris and Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X (2023) Metabolite profiling of compounds from Sargassum polycystum using UPLC-QToF-MS/MS. Pharmacognosy Journal, 15 (3). pp. 321-333. ISSN 09753575

[img]
Preview
Text
15422.pdf - Published Version
Available under License Creative Commons Attribution.

Download (786kB) | Preview
Full text available at: https://www.phcogj.com/article/2038

Abstract

Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles.
Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment.
Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC® H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases.
Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 % ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds.
Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.

Item Type: Journal Article
Keywords: metabolite profiling; Sargassum polycystum; seaweed; UPLC-QToF-MS/MS
Subjects: 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Burhan Ma'arif
Date Deposited: 29 Aug 2023 08:43

Downloads

Downloads per month over past year

Loading...

Origin of downloads

Loading...

Actions (login required)

View Item View Item
Sorry the service is unavailable at the moment. Please try again later.