Riwanti, Pramudita, Prasetyanti, Intan Kris and Ma'arif, Burhan ORCID: https://orcid.org/0000-0001-9182-343X (2023) Metabolite profiling of compounds from Sargassum polycystum using UPLC-QToF-MS/MS. Pharmacognosy Journal, 15 (3). pp. 321-333. ISSN 09753575
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Abstract
Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles.
Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment.
Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC® H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases.
Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 % ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds.
Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.
Item Type: | Journal Article |
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Keywords: | metabolite profiling; Sargassum polycystum; seaweed; UPLC-QToF-MS/MS |
Subjects: | 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences |
Divisions: | Faculty of Medical and Health Sciences > Department of Pharmacy |
Depositing User: | Burhan Ma'arif |
Date Deposited: | 29 Aug 2023 08:43 |
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