Metabolite profiling of compounds from Sargassum polycystum using UPLC-QToF-MS/MS

Riwanti, Pramudita, Prasetyanti, Intan Kris and Ma'arif, Burhan ORCID: (2023) Metabolite profiling of compounds from Sargassum polycystum using UPLC-QToF-MS/MS. Pharmacognosy Journal, 15 (3). pp. 321-333. ISSN 09753575

15422.pdf - Published Version
Available under License Creative Commons Attribution.

Download (786kB) | Preview
Full text available at:


Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles.
Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment.
Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC® H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases.
Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 % ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds.
Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.

Item Type: Journal Article
Keywords: metabolite profiling; Sargassum polycystum; seaweed; UPLC-QToF-MS/MS
Subjects: 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Burhan Ma'arif
Date Deposited: 29 Aug 2023 08:43


Downloads per month over past year

Origin of downloads

Actions (login required)

View Item View Item