Induction of synthetic polyploids of Porang (Amorphophallus muelerri blume) and assessment of its genetic variability using morphological data and RAPD molecular marker

Suyono, Suyono, Indivia, Imey Tamara ORCID: https://orcid.org/0000-0001-6623-5257, Resmisari, Ruri Siti, Fitriyah, Fitriyah and Wahyudi, Didik (2023) Induction of synthetic polyploids of Porang (Amorphophallus muelerri blume) and assessment of its genetic variability using morphological data and RAPD molecular marker. Journal of Tropical Biodiversity and Biotechnology, 8 (3). pp. 1-12. ISSN 2540-9581

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Abstract

This study uses morphological characteristics and RAPD markers to evaluate the polyploidization of synthetic porang. Seeds of triploid porang (2n=2x=26) were soaked in the different colchicine concentrations for 24 hours. After colchicine treatment, the porang seeds were planted to an MS medium that contained 2.2 µM of 6-benzylaminopurine (BAP), then, 40 days after planting in the MS media, the morphology and molecular of synthetic polyploid porang were characterized. For DNA extraction, a total of 100 mg of young leaves of porang plantlet was collected. One way Anova followed by the Duncan test (95%) was performed for phenotypic characterization. The number of different alleles, number of effective alleles, Shannon's information index, diversity, and unbiased diversity were assessed for genetic diversity. Synthetic polyploid porang has a higher total shoot, root, and wider leaves than normal porang. Polyploidy induction also successfully increased the genetic diversity of porang, and the genetic diversity will increase porang adaptability and sustainability of porang cultivation.

Item Type:	Journal Article
Keywords:	genetically modified organisms; iles-iles; molecular marker porang mutant; suweg
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	12 Oct 2023 10:40

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