Clinacanthus nutans L extracts reduce the serum tumor necrosis factor-α, malondialdehyde, and interleukin-6 levels and improve the Langerhans islet area in diabetic rat models

Mustika, Arifa, Fatimah, Nurmawati, Safitri, Indri, Susanti, Nurlaili ORCID: https://orcid.org/0000-0002-3799-2440 and Noor, Nurul Shahfiza (2023) Clinacanthus nutans L extracts reduce the serum tumor necrosis factor-α, malondialdehyde, and interleukin-6 levels and improve the Langerhans islet area in diabetic rat models. Clinical Medicine Insights: Endocrinology and Diabetes, 16. pp. 1-7. ISSN 11795514

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Abstract

Background:
Diabetes mellitus-induced hyperglycemia increases oxidative stress and inflammatory cytokine production, which play a significant role in the damage and apoptosis of pancreatic β cells. Therefore, the administration of medications that can reduce oxidative stress and inflammation plays an important role in diabetes treatment.
Objective:
To probe the Clinacanthus nutans leaf extract effect on oxidative stress and inflammatory markers and the Langerhans islet area in diabetic rat models.
Design:
An experimental laboratory in the animal model.
Methods:
Twenty-five diabetic rat models were randomly assigned into 5 clusters. Clusters 1, 2, and 3 were administered with C. nutans leaf extract in aqueous suspension with vehicle 1% Na-CMC at 75 mg/kg body weight (BW), 150 mg/kg BW, and 300 mg/kg BW, respectively. Cluster 4 was diabetic control rats administered with metformin at a 21 mg/rat dose. Cluster 5 was a control diabetic rat only administered with 1% Na-CMC suspension. Treatment was administered orally for 14 days. On the 15th day, the rats were sacrificed to obtain blood samples and pancreatic tissues. Serum interleukin (IL)-6, malondialdehyde (MDA), and tumor necrosis factor (TNF-α) were measured using the enzyme-linked immunosorbent assay (ELISA) method. Histopathological examination was performed by counting the Langerhans islet areas.
Results:
The average IL-6, MDA, and TNF-α levels declined in the cluster receiving C. nutans extract and were significantly different from the untreated cluster (P < .05). Histopathological examination revealed a significant upsurge in the Langerhans islets area in diabetic rats receiving C. nutans extract at doses of 75 and 150 mg/kg (P < .05).
Conclusion:
C. nutans leaf extract reduced the serum MDA, TNF-α, and IL-6 levels, and increased the Langerhans islets area in a diabetic rat model.
Keywords: Diabetes, medicine, tumor necrosis factor-α, malondialdehyde, interleukin-6, Langerhans islet, Clinacanthus nutans L

Item Type:	Journal Article
Keywords:	diabetes; medicine; tumor necrosis factor-α; malondialdehyde; interleukin-6; Langerhans islet; Clinacanthus nutans L
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User:	Nurlaili Susanti
Date Deposited:	02 Nov 2023 10:22

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