Elucidating the active compound profile and mechanisms of Dendrophthoe pentandra on colorectal cancer: LCMS/MS identification and network pharmacology analysis

Mutiah, Roihatul ORCID: https://orcid.org/0000-0002-8196-9029, Rachmawati, Ermin ORCID: https://orcid.org/0000-0003-1045-7066, Zahiro, Syayida Roisatus and Milliana, Alvi ORCID: https://orcid.org/0000-0003-1010-3591 (2024) Elucidating the active compound profile and mechanisms of Dendrophthoe pentandra on colorectal cancer: LCMS/MS identification and network pharmacology analysis. Journal of Applied Pharmaceutical Science, 14 (02). pp. 222-231. ISSN 2231-3354

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Abstract

Colorectal cancer (CRC) is the second leading cause of death worldwide, and its incidence has significantly increased over the past decade. Although the herbal plant Dendrophthoe pentandra (DPT) has been reported by previous researchers to possess CRC inhibition activity, the exact active compound profile, and mechanisms have not been fully elucidated. Therefore, this study aimed to determine the profile of the active compound and systematically explore the pharmacological and molecular mechanisms of DPT on CRC inhibition. The identification of compounds in this study was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the mechanism of action was determined using a network pharmacology approach, absorption, distribution, metabolism, excretion, target gene prediction, network analysis, and gene enrichment analysis. The results showed that 18 active compounds were identified using LC-MS/MS. Network analysis revealed that quercetin and Phyllanthusiin E modulated the target genes MYC, Caspase 3, Jun proto-oncogene, Mitogen-activated protein kinase 1, B-cell lymphoma 2, Mitogen-activated protein kinase 8, Bcl-2-associated X protein, and Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma. Gene enrichment analysis showed that DPT may be beneficial for CRC patients by modulating apoptosis, P53 signalling, Mitogen-activated protein kinase signalling, Wnt signalling, and Phosphatidylinositol 3-kinase-protein kinase B signalling pathways. This study partially demonstrated and predicted the pharmacological and molecular mechanisms of DPT on CRC from a holistic perspective; therefore, DPT can be recommended for further research in developing anti-CRC drugs.

Item Type:	Journal Article
Keywords:	Dendrophthoe pentandra; colorectal cancer; LC-MS/MS; network pharmacology
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111201 Cancer Cell Biology 11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics 11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Dr. M.Kes. Roihatul Muti'ah
Date Deposited:	15 Feb 2024 14:18

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