Identification of active compounds Calotropis gigantea stem extracts as antimalarials agents on animal infected Plasmodium berghei

Muhammad, Sofiyullah and Mutiah, Roihatul and Hayati, Elok Kamilah and Idradmojo, Christyaji (2016) Identification of active compounds Calotropis gigantea stem extracts as antimalarials agents on animal infected Plasmodium berghei. Journal of Islamic Pharmacy, 1 (1). pp. 12-19. ISSN 2527-6123

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Abstract

Thistle (Calotropisgigantea) is a wild plant that contains secondary metabolites as a potential antimalarial drugs.This study aims to determine the content of active compound contained, the potential of ethanol extract of the stem thistle as an antimalarial, and knowing the best eluent used when TLC test.This study covers the extraction of stem thistle using maceration for 24 hours using ethanol 80%.Stirring assisted with shaker for 3 hours. Ekstrak concentrated phytochemical test, antimalarial activity was evaluated by experimental animals mice infected by Plasmodium berghei.Data degree of parasitaemia in mice were analyzed using Minitab 16 with Test TwoWay ANOVA followed by Tukey test.The results showed that ethanol extract stem thistle contains the active compound class of triterpenoids, and potentially as an antimalarial with ED50 values 152.878 mg / kg bw were included in both categories.TLC test results showed that the best eluent for the separation of triterpenoids of stem thistle extract is benzene: chloroform (3: 7) which produced 12 spots with Rf values of 0.04;0.06;0.14;0.21;0.29;0.35;0.41;0.48;0.80;0.84;0.88;0.91.

Item Type: Journal Article
Keywords: Antimalarial; Calotropisgigantea; Ethanol 80%; Plasmodium berghei; Thin Layer Chromatography
Subjects: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules
03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030499 Medicinal and Biomolecular Chemistry not elsewhere classified
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Dr. M.Kes. Roihatul Muti'ah
Date Deposited: 27 Apr 2017 10:29

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