Genetic Diversity and Relationship among and within the Bird of Paradise Plants of Strelitziaceae and Heliconiaceae

Kholqiyah, Siti Faiqotul, Wahyudi, Didik and Hapsari, Lia (2024) Genetic Diversity and Relationship among and within the Bird of Paradise Plants of Strelitziaceae and Heliconiaceae. Jordan Journal of Biological Sciences, 17 (1). pp. 163-171. ISSN 19956673, 23077166

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Abstract

Bird of paradise plants belong to families Strelitziaceae and Heliconiaceae, two close-related families in the Zingiberales order. This study aimed to study the genetic diversity and relationship of species among and within Strelitziaceae and Heliconiaceae using molecular RAPD markers and compare them to morphological characters. Samples used comprised two species from Strelitziaceae and eight species from Heliconiaceae. Twenty OPA primers showed that 17 out of 20 OPA primers were successfully amplified. Primer OPA-10 was the most informative and recommended, followed by OPA-5, OPA-7, OPA-9, OPA-11, OPA-17, OPA-18, OPA-19, and OPA-20. Heliconiaceae and Strelitziaceae were high genetic diversity (P=100%, I=0.388±0.016, He=0.237±0.013) with low similarities (0.09-0.39). Heliconiaceae was more genetically diverse than Strelitziaceae. Heliconiaceae produced P=98.88%, I=0.409±0.017, and He=0.254±0.014. Strelitziaceae
produced P=60.53%, I=0.366±0.049, and He=0.251±0.033. The tree topology based on the RAPD marker differs slightly
from morphology. However, the morphological characteristics are important to support and complement it. Strelitziaceae
was strongly separated from Heliconiaceae, which supports the monophyly of both families. Heliconiaceae was separated
into two subclusters and paraphyletic. Subcluster 1 comprises subgenera Heliconia and Stenochlamys. Subcluster 2
comprises subgenera Griggsia and Stenochlamys. In conclusion, the molecular RAPD marker proved the powerful of
classification at the family level but moderate at subgenus, section, and species levels. Nonetheless, it is considered a simple and valuable method to provide a primary reference for taxonomic delimitation, conservation and breeding efforts of the bird of paradise plants in the future.

Item Type:	Journal Article
Keywords:	RAPD; phylogenetic; polymorphism; molecular taxonomy; variability
Subjects:	06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	05 Apr 2024 11:10

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