Effect of pegagan (Centella asiatica) nanoparticle coated with chitosan on the cytokineprofile of chronic diabetic mice

Muchtaromah, Bayyinatul ORCID: https://orcid.org/0000-0001-9968-8295, Firdaus, Ana Mar'a Konita, Ansori, Arif Nur Muhammad, Duhita, Maharani Retna ORCID: https://orcid.org/0000-0002-1651-2903, Minarno, Eko Budi ORCID: https://orcid.org/0000-0003-0793-4802, Hayati, Alfiah, Ahmad, Mujahidin and Analisa, Nur Izzah (2024) Effect of pegagan (Centella asiatica) nanoparticle coated with chitosan on the cytokineprofile of chronic diabetic mice. Narra J, 4 (1). pp. 1-15. ISSN 28072618

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Abstract

Diabetes is closely related to immune response problems when it occurs chronically. Pegagan (Centella asiatica)is a medicinal plant with active compounds.Madecassosideis beneficial in treating diabetes,andnanoparticle technology is expected to enhance the medicinal potential and availability of pegagan compounds. The aim of this study was to determine the effect of chitosan-coated pegagan nanoparticles on the cytokine profile of chronic diabetic mice,which includedCD4+TNF-α+, CD8+TNF-α+,CD4+IFN-γ+,CD8+IFN-γ+and IL-6+. An experimentalstudywitharandomizedcomplete block design (CRD) consistingof six treatments with seven replicateswas conducted. The groups were: healthy mice as negative control; diabetic mice treated with distilled wateras positive control and diabetic mice treated withnanoparticle coated with chitosan (NPC) 20 mg/kg,30 mg/kg, 40 mg/kg, and metformin 130 mg/kgBW. The data were tested using one-way analysis of variance (ANOVA) with a significance level of 5% and continued with the Duncan’smultiple range test. The results showed that pegaganNPC could significantly reduce therelative numberof CD4+TNF-α+, CD8+TNF-α+, CD4+IFN-γ+and CD8+IFN-γ+and IL-6 in the dose of 20 mg/kg, 30 mg/kg and 40 mg/kg(p<0.05). The treatment dose of 20 mg/kg reducedCD4+TNF-α+, CD8+TNF-α+, CD4+IFN-γ+, CD8+IFN-γ+to the levels of healthy miceand a dose of 30 mg/kg couldreduce IL-6 as in healthy mice. These findings suggest that chitosan-coated pegagan nanoparticles area promisingtherapy for diabetes,as they have the potential to modulate the immune response associated with chronic diabetes

Item Type:	Journal Article
Keywords:	Diabetes mellitus; immune system; antidiabetic; Centella asiatica; nanomedicine
Subjects:	06 BIOLOGICAL SCIENCES > 0608 Zoology > 060802 Animal Cell and Molecular Biology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Maharani Retna Duhita
Date Deposited:	26 Apr 2024 10:33

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