RAPD analysis to reveal the genetic diversity among closely related Etlingera species of Achasma group, Zingiberaceae

Trimanto, Trimanto, Probojati, Rasyadan Taufiq, Wahyudi, Didik and Hapsari, Lia RAPD analysis to reveal the genetic diversity among closely related Etlingera species of Achasma group, Zingiberaceae. Presented at International Conference on Environmental, Mining, and Sustainable Development 2022.

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Abstract

The morphological characteristics among closely related Etlingera species of Achasma group were found overlapping which needs to be confirmed molecularly. Hence, the purposes of this study were to reveal the genetic diversity and relationship of four species, namely E. megalocheilos, E. littoralis, E. coccinea and E. metriocheilos using RAPD
markers, also to differentiate the species identity of E. cf. megalocheilos specimens from Sulawesi and Java. The results showed that 13 out of 20 OPA primers were successfully amplifying the DNAs target. Five highly polymorphic primers were selected as recommended markers i.e. OPA09, OPA10, OPA11, OPA18, and OPA20. Diversity analysis showed high genetic richness with Shannon index of 0.417 and the number of polymorphic loci of 98.90%. Clustering and PcoA analyses resulted in three clusters following their species identity. Cluster 1 comprised of three specimens of E. littoralis (similarity value of 44.44% to 61.79%). Cluster 2 comprised of E. coccinea and E. metriocheilos (similarity value of 40.32%). Cluster
3 comprised of E. cf. megalocheilos Sulawesi and E. megalocheilos from Java (similarity value of 34.78%). This study has confirmed that the four Etlingera spp. were genetically distinct, and E. cf. megalocheilos from Sulawesi and Java were possibly the same species but still unclear. Further research using DNA barcodes for a more precise identification is required

Item Type:	Conference (Paper)
Keywords:	Etlingera; Molecular; RAPD; Polymorphism; Relationship; Zingiberaceae
Subjects:	06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060311 Specialisation and Extinction 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	29 Apr 2024 09:43

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