Aktivitas antibakteri dan antibiofilm nanopartikel Ag/N-TiO2 terhadap multidrug resistant klebsiella pneumoniae pada material kateter silikon (sertifikat hak cipta)

Alif Firman Firdausy (2024) Aktivitas antibakteri dan antibiofilm nanopartikel Ag/N-TiO2 terhadap multidrug resistant klebsiella pneumoniae pada material kateter silikon (sertifikat hak cipta). 000586871.

Text 18987.pdf Download (2MB)

Abstract

The rise of drug-resistant bacterial strains is escalating due to the ability to produce biofilms shielding bacteria from antimicrobial agents. Consequently, novel approaches are imperative for managing biofilm-related infections in healthcare settings. Silver based nanoparticles have revealed potential antibacterial characteristic against various bacteria. In the present work, silver-doped TiO2 (Ag-TiO2), silver/Nitrogen co-doped TiO2 (Ag/N-TiO2) were synthesized using sol gel and photochemical deposition under UV illumination. FTIR, XRD, DRS were performed to characterize the vibrational, structural, and optical properties, respectively. In addition, FE-SEM and EDX analysis were also utilized to determine the surface morphology, particle size, and elemental composition of the prepared materials. Furthermore, the synthesized Ag-TiO2 and Ag/N-TiO2 were explored and compared as antimicrobial and antibiofilm agents against clinically isolated multidrug-resistant (MDR) Klebsiella pneumoniae (K. pneumoniae) on the silicone rubber as a urinary catheter material in the medical devices. Various silver concentrations (10-1,10-2, and 10-3 M) on the TiO2 and N-TiO2 were assessed for their antimicrobial effects and anti-biofilm properties against MDR K. pneumoniae. Through the disc diffusion method, it was found that the 10-3M silver concentration on Ag-TiO2 nanoparticles represented the highest inhibition zone (77.29%) against MDR K. pneumoniae. Antibiofilm assay through the crystal violet method revealed that Ag-TiO2 with 10-1M silver concentration inhibited 54.20% of biofilm formation. In conclusion, Ag-TiO2 and Ag/N-TiO2 nanoparticles have exhibited a promising antibacterial and antibiofilm agents in medical devices, providing an effective inhibition toward the bacterial growth and biofilm formation of MDR K. pneumoniae.

Item Type:	Hak Cipta
Keywords:	Antibiofilm; Ag/N-TiO2; nanopartikel
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Alif Firman Firdausy
Date Deposited:	15 May 2024 14:19

Downloads

Origin of downloads

Actions (login required)

View Item