Antioxidant, anti-collagenase, and antibacterial activities of Fucus vesiculosus silver nanoparticles

Savitri, Evika Sandi, Rahmawaty, Annisa E. and Minarno, Eko Budi ORCID: https://orcid.org/0000-0003-0793-4802 (2024) Antioxidant, anti-collagenase, and antibacterial activities of Fucus vesiculosus silver nanoparticles. Narra J, 4 (3). pp. 1-11. ISSN 28072618

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Abstract

Fucus vesiculosusis an algawith high fucoxanthin, phlorotannin, fucoidan, sterol, and astaxanthin. The silver nanoparticles of F. vesiculosus(AgNPs-Fv) are expected to have high antioxidant, anti-collagenase, and antibacterial activities. The aim of this study was to characterize the distribution and size of AgNPs-Fvand determine theirantioxidant, anti-collagenase, and antibacterial activities. The distribution and size of AgNPs-Fvwere measured using particle size analyzer (PSA) analysis. The nanoparticle compound and their functional groups were characterized using a scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR), respectively. The antioxidant activity of AgNPs-Fv was determined using a 1.1-diphenyl-2-picrylhydrazyl(DPPH) assay, while the anti-collagenaseactivity was examined using thespectrophotometric method. The antibacterial activity was assessed using an inhibition zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Results indicated that the AgNPs-Fvhada dominant volume of about 86.3% with a diameter of 113.3 nm. SEM analysis revealed the spherical AgNPs with sizes between 27 and 54 nm. The FTIR analysis of the AgNPs-Fv absorption band at 1,046 cm-1demonstratedthe bond between the Ag metal and the O-H hydroxyl group. The antioxidant activity of AgNPs-Fv was higher than F. vesiculosus extract(24.23±3.55 mg/L vs 47.45±3.16 mg/L). AgNPs-Fv also had a higheranti-collagenaseactivity compared to F. vesiculosusextract (66.74±6.352 mg/L vs 145.1±6.326 mg/L). The inhibition zone diameter of AgNPs-Fv was greater than F. vesiculosusextract. The MIC and MBC of AgNPs-Fv were 18.75 and 18.75 ppm, while F. vesiculosusextract was 37.5 and 75 ppm, respectively. These results suggested that AgNPs of F. vesiculosushad higher antioxidant, anti-collagenase, and antibacterial activitiesthan F. vesiculosusextractalone

Item Type:	Journal Article
Keywords:	Fucus vesiculosus; AgNPs; antibacterial; anti-collagenase; antioxidan
Subjects:	06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Sandi Savitri
Date Deposited:	29 Nov 2024 12:39

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