Pengaruh ekstrak fermentasi sambal lalapan terhadap ekspresi gen IL-6 dan TNF-α pada hepar kelinci model Non Alcoholic Fatty Liver Disease (NAFLD)

Rachmawati, Ermin ORCID: https://orcid.org/0000-0003-1045-7066, Kinasih, Larasati Sekar ORCID: https://orcid.org/0000-0003-3259-9364, Syauki, Moh.Faris, Fahdela, Ahdika Nungki, Putri, Fayza Cinta R and Akbar, Iqbal Maulana (2024) Pengaruh ekstrak fermentasi sambal lalapan terhadap ekspresi gen IL-6 dan TNF-α pada hepar kelinci model Non Alcoholic Fatty Liver Disease (NAFLD). Research Report. Fakultas Kedokteran dan Ilmu Kesehatan UIN Maulana Malik Ibrahim Malang. (Unpublished)

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Abstract

Non alcoholic Fatty Liver Disease (NAFLD) is a chronic liver damage disease without significant alcohol intake, characterized by inflammation and steatosis of the liver. Until now there is no specific therapy for NAFLD, statin intervention as NAFLD therapy has not been able to provide optimal results and cause side effects in the form of hepatotoxicity and myopathy. Objective: To determine the effect of Vegetable Spices Fermentation Extract (VSFE) extract on hepatic PPAR-γ mRNA gene expression in NAFLD model rabbits fed a high-fat diet.

Methods: This study was a pure experimental study using a post test only control group design. 24 rabbits were divided into 4 random groups, namely the normal group (without treatment), the negative control group (group given a high fat diet), treatment group 1 (group given a high-fat diet and VSFE dose of 100mg/kgBB), and treatment group 2 (group given a high-fat diet and VSFE dose of 200mg/kgBB). The relative gene expression of PPAR-γ mRNA was evaluated using PCR and the Livak 2 method formula. Results: The results showed that there was a significant difference in PPAR-γ mRNA gene expression. EFSL doses of 100mg/kgBB and 200 mg/kgBB decreased PPAR-γ expression by 1.19 ± 0.34 and 1.29± 0.63 compared to the control group of 2.29 ± 0.76 (p<0.05). Conclusion: VSFE is able to prevent NAFLD by inhibiting inflammation through suppression of PPARγ expression.-ΔΔCT

Item Type:	Research (Research Report)
Keywords:	Vegetable Spices Fermentation; PPAR-γ; NAFLD; Steatosis
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User:	Larasati Sekar Kinasih
Date Deposited:	09 Dec 2024 13:33

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