Wahyudi, Didik, Putri, Amalia Nasyira, Resmisari, Ruri Siti and Suyono, Suyono (2024) Genetic variation of Porang (Amorphophallus muelleri Blume) After Mutation Induction Using Colchicine. Presented at International Conference on Green Technology.
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Abstract
Porang (Amorphophallus muelleri Blume) is a tuberous plant with the highest glucomannan among Amorphophallus genera. However, the low genetic diversity of porang today can affect porang cultivation in the future. Therefore, increasing the genetic diversity of porang by polyploidy induction using colchicine is needed. 30 days after planting porang shoot explants were used for polyploidy induction. After polyploidy induction using colchicine, DNA was extracted using Geneid Genomic DNA Mini Kit. Genetic diversity and clustering analysis was performed to assess the genetic diversity of porang after mutation induction. Mutation induction using colchicine successfully increased the genetic diversity of porang. Polyploidy induction using 0.05% colchicine is recommended to increase genetic variation of porang.
| Item Type: | Conference (Keynote) |
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| Keywords: | Amorphophallus Muelleri Blume, Genetic Diversity, Issr, Mutation, Polyploidy |
| Subjects: | 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 06 BIOLOGICAL SCIENCES > 0606 Physiology 06 BIOLOGICAL SCIENCES > 0607 Plant Biology |
| Divisions: | Faculty of Mathematics and Sciences > Department of Biology |
| Depositing User: | Didik Wahyudi |
| Date Deposited: | 22 Jan 2025 14:17 |
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