Colchicine-induced genetic variability in porang (Amorphophallus muelleri): A study using SRAP markers

Wahyudi, Didik, Suyono, Suyono, Resmisari, Ruri Siti and Mauludyah, Rohmatul (2025) Colchicine-induced genetic variability in porang (Amorphophallus muelleri): A study using SRAP markers. Biotropika: Journal of Tropical Biology, 13 (2). pp. 73-82. ISSN 2549-8703 (e.); 2302-7282 (p.)

Text 24272.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (665kB)

Abstract

Mutation induction of porang both physically and chemically is widely used to increase the genetic variation of porang. However, mutation induction chemically by using colchicine is more effective than the other mutagens. Therefore, this study aims to assess the genetic di-versity of porang after mutation induction using colchicine. Polyploidy induction is per-formed 30 days after planting of the porang plantlet. 0%, 0.01%, 0.02%, 0.03% and 0.04% colchicine was added to 12.5 ml of MS medium using a 0.22 µm Millipore filter. After 53 days, porang plantlets were ready for DNA isolation. A total of 10 SRAP primer combina-tions were used for DNA amplification. Genetic diversity in and between porang population was performed using POPGENE software. Clustering analysis using PAST Software was also performed to identify genetic variation. Mutation induction using colchicine significant-ly increased the genetic diversity of porang. Among the SRAP primer combinations, ME2-EM2 is the most effective in detecting the induced genetic variation in porang. Mutation in-duction using 0.03% colchicine is highly recommended as it produces significant genetic changes compared to normal porang. Porang mutant resulting from 0.03% colchicine treatment holds great potential for porang breeding programs, as it can generate novel phenotypic variations and increase the chances of obtaining superior genotypes with desir-able agronomic traits.

Item Type:	Journal Article
Keywords:	colchicine; genetic variation; mutation induction; porang
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060408 Genomics 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	19 Aug 2025 13:01

Downloads

Origin of downloads

Actions (login required)

View Item