Upregulation of Glucomannan Synthesis Genes in Porang (Amorphophallus muelleri Blume) after Polyploidy Induction using Colchicine (sertifikat hak cipta)

Didik Wahyudi, MSi., Suyono MP., Ruri Siti Resmisari, M.Si (2025) Upregulation of Glucomannan Synthesis Genes in Porang (Amorphophallus muelleri Blume) after Polyploidy Induction using Colchicine (sertifikat hak cipta). EC002025180517.

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Abstract

Inducing polyploidy in porang using colchicine has been explored and successfully enhanced the genetic variability of porang. However, the specific impact of polyploidy on the expression of the genes responsible for glucomannan synthesis in porang has not been extensively studied. Therefore, this study aims to evaluate the effect of polyploidy induction on the expression of genes responsible for glucomannan synthesis in porang. Porang shoot explants (2n = 26) were used for polyploidy induction. Polyploidy induction was carried out using colchicine added to the culture medium. The concentrations of colchicine used in this study were 0 %, 0.01 %, 0.02 %, 0.03 %, and 0.04 %. After 53 days in media without colchicine, explants were collected for flow cytometry analysis and RNA isolation. The application of colchicine at various concentrations induced tetraploid and mixoploid porang. Mutation induction using colchicine also increases the expression (upregulation) of the CSLA, GMPP, CSLD, and GMPP genes encoding glucomannan biosynthesis in porang. Tetraploid (0.01 % colchicine treatment) and mixoploid porang (0.02 % colchicine treatment) showed the highest expression of genes involved in glucomannan biosynthesis via both sucrose and invertase pathways.

Item Type:	Hak Cipta
Keywords:	Colchicine, glucomannan biosynthesis, polyploid porang, porang cultivation.
Subjects:	06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 06 BIOLOGICAL SCIENCES > 0607 Plant Biology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	21 Nov 2025 18:32

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