In silico assessment of anti-biofilm properties of bioactive compounds from Parameria laevigata (Juss.) Moldenke identified by UPLC QToF MS/MS

Anggraini, Wirda ORCID: https://orcid.org/0000-0002-9837-155X, Purwanto, Djoko Agus ORCID: https://orcid.org/0000-0002-2718-903X, Kusumawati, Idha ORCID: https://orcid.org/0000-0002-9451-3098 and Isnaeni, Isnaeni ORCID: https://orcid.org/0000-0003-4502-2433 (2026) In silico assessment of anti-biofilm properties of bioactive compounds from Parameria laevigata (Juss.) Moldenke identified by UPLC QToF MS/MS. Journal of Medicinal and Pharmaceutical Chemistry Research, 8 (3). pp. 729-738. ISSN 2981-0213

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Abstract

Parameria laevigata (Juss.) Moldenke is a plant with the potential to act as an antifungal agent against various diseases, including vaginitis. This is primarily due to quinones, flavonoids, saponins, triterpenoids, tannins, alkaloids, polyphenols, and steroids in the bark. Vaginitis is a condition caused by Candida albicans fungal through biofilm formation. The attachment of the fungal to epithelial cells and subsequent invasion of host cells often require the presence of Als3p, which can also serve as a receptor in silico. Therefore, this study aims to determine compounds of P. laevigata that are associated with Als3p and possess anti-biofilm properties in silico. The ultrasound-assisted Extraction (UAE) method was used for extraction, while the UPLC-QToF-MS/MS instrument was used to determine the metabolite profile. MOE software determined molecular docking between Als3p (PDB id: 4LEE) and metabolite profiling. MassLynx software was used for the data interpretation, and confirmation was performed on various websites, including MassBank, NIST, PUB-CHEM, HMDB, MoNA, and DrugBank. The results showed that P. laevigata stem bark ethanol extract contained 19 known compounds. In addition, 3 compounds were found to possess antimicrobial activity, including myristicin, valyl phenylalanine, and oleanane. In silico investigation revealed that myristicin, valyl phenylalanine, and oleanane could inhibit Als3p receptor. Based on the results, P. laevigata stem bark ethanol extract contained valyl phenylalanine, myristicin, and oleanane, indicating anti-biofilm activity. Myristicin, valyl phenylalanine, and oleanane were also shown to have the ability to inhibit the Als3p receptor.

Item Type:	Journal Article
Keywords:	Als3p; anti-biofilm; medicine; metabolite profile; Parameria laevigata; UPLC-QToF-MS/MS
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Wirda Anggraini
Date Deposited:	15 Apr 2026 09:50

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