Molecular Detection of the Plantaricin A (plnA) Gene in the Lactobacillus casei Group

Kusmiyati, Nur ORCID: https://orcid.org/0000-0003-3368-5723, Utami, Ulfah ORCID: https://orcid.org/0000-0002-9811-5759, Kusnadi, Joni and Septiana, Siska (2025) Molecular Detection of the Plantaricin A (plnA) Gene in the Lactobacillus casei Group. Molecular Detection of the Plantaricin A (plnA) Gene in the Lactobacillus casei Group, 15 (10.332). pp. 1-11. ISSN 2069-5837

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Abstract

The plantaricin A (plnA) gene encodes a pheromone peptide that induces the synthesis of
bacteriocins in Lactobacillus plantarum but can also be found in other species. Lactobacillus casei,
Lactobacillus paracasei, and Lactobacillus rhamnosus are the most commonly studied and used
probiotics for the synthesis of antimicrobial compounds such as bacteriocins. This study specifically
focused on detecting the presence of the plnA gene, a single gene associated with plantaricin synthesis,
in the L. casei group. Detection of the plnA gene was carried out through bacterial culturing, direct
colony PCR, PCR amplification using plnA-F and plnA-R specific primers, electrophoresis,
sequencing, and sequence analysis using BLAST. The presence of bands in the electrophoresis of PCR
results revealed that the plnA gene was not detected in L. rhamnosus, but was found in L. casei and L.
paracasei. Sequencing analysis of the plnA gene from L. casei and L. paracasei revealed 99.56% and
100% similarity with the plnA gene from L. plantarum EG.LP.18, respectively. The plnA gene found
in Lactobacillus casei and Lactobacillus paracasei can be used to produce bacteriocins, which are
antibacterial compounds.

Item Type:	Journal Article
Keywords:	anti-bacterial agents; bacteriocins; Lactobacillus casei; plnA gene
Subjects:	06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Ulfah Utami
Date Deposited:	15 Jan 2026 14:25

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