Cellular uptake and nuclear accumulation of polystyrene nanoplastics in 3T3 fibroblasts and hepatocytes of rattus norvegicus

Saputra, Febriyansyah, Hayati, Alfiah, Septiani, Adiibtia, Pramudya, Manikya, Susilo, Raden Joko Kuncoroningrat, Lim, Vuanghao and Muchtaromah, Bayyinatul ORCID: https://orcid.org/0000-0001-9968-8295 (2025) Cellular uptake and nuclear accumulation of polystyrene nanoplastics in 3T3 fibroblasts and hepatocytes of rattus norvegicus. Advances in Animal and Veterinary Sciences, 13 (7). pp. 1491-1501. ISSN 2307-8316

Preview

Text 28413.pdf Download (2MB) | Preview

Abstract

Polystyrene nanoplastics (PSNPs) are emerging contaminants that pose potential risks to cellular health due
to their small size and ability to penetrate biological barriers. This study investigates the cellular uptake mechanisms and
intracellular distribution of PSNPs using Rattus norvegicus hepatocytes and 3T3 fibroblast cell cultures. Fluorescence
microscopy analysis demonstrated substantial PSNP accumulation within both cell types, with distinct intracellular
localization patterns. In hepatocytes, PSNPs were predominantly observed in the cytoplasm and occasionally within the
nucleus, suggesting active endocytosis and potential nuclear transport. In 3T3 fibroblasts, PSNPs were found not only
in the cytoplasm and around the nucleus but also within the nuclear compartment, highlighting their ability to traverse
the nuclear envelope. Quantitative analysis revealed significant morphological changes, increased cell and nucleus diam
eters, and a higher percentage of necrotic cells in PSNP-exposed groups compared to controls (p < 0.05). The observed
cellular stress responses may induce oxidative stress, membrane disruption, and apoptosis, supported by morphological
and fluorescence evidence indicating interactions with proteins, lipids, and nuclear components.. These findings high
light the potential health risks associated with PSNP exposure, highlighting the need for further research into their
long-term biological impacts and the mechanisms underlying their cellular interactions.

Item Type:	Journal Article
Keywords:	nanoplastics; cellular health; cellular-transport; hepatocytes; 3t3-fibroblast
Subjects:	06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism
Divisions:	Graduate Schools > Magister Programme > Graduate School of Biology
Depositing User:	Bayyinatul Muchtaromah
Date Deposited:	21 Jul 2026 10:35

Downloads

Origin of downloads

Actions (login required)

View Item