Metabolite profiling of porang (Amorphophallus muelleri Blume) after Polyploidization using Colchicine

Wahyudi, Didik, Suyono, Suyono, Resmisari, Ruri Siti ORCID: https://orcid.org/0000-0001-6623-5257, Prateso, Bagas and Yusnawan, Eriyanto (2026) Metabolite profiling of porang (Amorphophallus muelleri Blume) after Polyploidization using Colchicine. AGRIVITA Journal of Agricultural Science, 48 (3). pp. 1-12. ISSN 24778516

Text 28957.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (1MB)

Abstract

Both chemically and physically, mutation induction is currently being intensively carried out to increase the genetic diversity of porang. However, in-depth evaluation of changes in secondary metabolites after mutation induction has not been widely implemented. Therefore, this study aims to classify the profile of secondary metabolites of porang after colchicine mutation induction and analyze the relationship between these metabolites and the glucomannan synthesis pathway. Porang explants induced with a variation of colchicine concentration were used for profiling metabolite analysis. The metabolite profile was identified using GC-MS (Gas chromatography tandem-mass spectrometry) and then analyzed using MetaboAnalyst. The construction of the metabolite pathway network was carried out using Cytoscape software. 0.02% of colchicine resulted mixoploid porang and has a different metabolite profile than normal porang. In addition, mixoploid porang (0.02% colchicine) has more metabolite content related to glucomannan synthesis. Therefore, mutation induction using a colchicine concentration of 0.02% is recommended to produce superior porang.

Item Type:	Journal Article
Keywords:	metabolite profiling; mutation induction; mutant porang; porang cultivation
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060408 Genomics 07 AGRICULTURAL AND VETERINARY SCIENCES > 0701 Agriculture, Land and Farm Management > 070108 Sustainable Agricultural Development 07 AGRICULTURAL AND VETERINARY SCIENCES > 0706 Horticultural Production > 070601 Horticultural Crop Growth and Development 07 AGRICULTURAL AND VETERINARY SCIENCES > 0701 Agriculture, Land and Farm Management 07 AGRICULTURAL AND VETERINARY SCIENCES > 0706 Horticultural Production
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	21 Sep 2026 10:55

Downloads

Origin of downloads

Actions (login required)

View Item