Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species

Milliana, Alvi and Noorhamdani, A.S. and Poeranto, Sri and Handono, Kusworini and Prawiro, Sumarno Reto and Fitrianingsih, Avin Ainur and Rachma, Lailia Nur (2017) Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species. Tropical Journal of Pharmaceutical Research, 16 (2). pp. 255-261. ISSN 1596-5996

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Abstract

Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species.

Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili using a pili bacterial cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with antibodies was examined by Western blotting.

Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.

Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.

Item Type: Journal Article
Keywords: Shigella flexneri OMP; Outer membrane protein; Protein adhesion; Antibody; Cross-reaction
Divisions: Faculty of Medical and Health Sciences > Department of Medical Education
Depositing User: Faizuddin Harliansyah
Date Deposited: 07 Jun 2018 05:42

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