Using an electrochemical assay to determine the biofilm elasticity change as a response to toxicant exposure

Daryono, Retno Novvitasari Hery, Feurtet-Mazel, Agnes, Bouletreau, Stephanie and Garabetian, Frederic (2018) Using an electrochemical assay to determine the biofilm elasticity change as a response to toxicant exposure. Presented at International Conference on Frontier of Science and Society 2017, 3-5 November 2017, Makassar.

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Elasticity is a trait of biofilm physiognomy which relates to cell clustering and can be measured by means of an electrochemical assay based on rotating disc electrode (RDE). This study aimed at testing the hypothesis according to which exposure of phototrophic biofilm to toxicant could reduce its elasticity. We compared biofilms developed for 21 days, in four sets of 6 replicated experimental units, in absence and presence of isoproturon at two concentrations of the inoculating suspension of biofilm, 103 and 104 diatom cell mL-1. Biofilm thickness and elasticity were measured based on RDE assay, bacterial and diatom density were measured by microscope-based numerations.Very thin biofilms (< 10 µm) were obtained as compared with a previous study. This might be linked with the way we selected the initial biofilm providing the suspension and the way we developed its growth. The biofilm elasticity mean values in the presence of isoproturon was quasi twice lower (60 ± 10 and 60 ± 41 µm rpm0.5) than the treatment without isoproturon (138 ± 93 and 115 ± 104 µm rpm0.5), for initial biofilm concentration of 103 and 104 respectively, but there was no significant difference between the mean values of each treatment. Nevertheless, the present preliminary study demonstrated the feasibility of an experiment dedicated to assessing biofilm elasticity changes as a response to toxicant exposure.

Item Type: Conference (Paper)
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Faizuddin Harliansyah
Date Deposited: 07 Jun 2018 09:54


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