Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing

Savitri, Evika Sandi (2013) Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing. American Journal of Molecular Biology, 3. pp. 32-37.

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Abstract

Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse phy- sicological and molecular responses in plants. These responses include changes in gene expression. One of drought tolerance gene is a gene encoding dehydrin which is belongs to the group II or D-11 LEA protein family. LEA-D11 gene produce dehydrin protein which has a role in stabilization of membrane struc- tures and protection of macromolecules in the pres- ence of drought. The aims of the study was to identify and to characterize the LEA-D11 gene in various soy- bean varieties. This research used seven varieties of soybean: Tanggamus, Nanti, Seulawah, Tidar (drought tolerant), Wilis and Burangrang (drought moderate) and Detam-1 (drought susceptible). DNA genome of those varieties was isolated using the methods from Doyle & Doyle [1]. DNA amplification was conducted using Polymerase Chain Reaction (PCR) with specific primers designed based on GmLEA-D11 gene sequence database from the NCBI. The DNA targets were se- quenced using automatic sequencing machine, ABI 3130xl Genetic Analyzer, in Eijkman Institution. The result of this study showed that the sequences of Gm- LEA-D11 gene possessed by drought tolerance varie- ties (Tanggamus, Nanti, Seulawah and Tidar) and moderately tolerance (Wilis and Burangrang) were similar. However, the sequence of GmLEA-D11 gene detected in the drought susceptible variety Detam-1 was different from the two groups. Similarity be- tween drought tolerance and moderately tolerance indicate that there is not only LEA-D11 gene respon- sible to drought tolerance but also others. The primer and sequences GmLEA-D11 gene can be used as mol- ecular marker and capable of differentiating between drought susceptible and drought moderate to drought

Item Type:	Journal Article
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Sandi Savitri
Date Deposited:	29 Apr 2016 08:18

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