Protein profiles and dehydrin accumulation in some soybean varieties (Glycine max L. Merr) in drought stress conditions

Arumingtyas, Estri Laras and Savitri, Evika Sandi (2013) Protein profiles and dehydrin accumulation in some soybean varieties (Glycine max L. Merr) in drought stress conditions. American Journal of Plant Sciences, 4. pp. 134-141.

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Abstract

Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesian soybeans: Tangga-mus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam-1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti, Seulawah and Tidar varie-ties. Western blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of dehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.

Item Type: Journal Article
Subjects: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Sandi Savitri
Date Deposited: 29 Apr 2016 08:18

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