Activity of 96% ethanol extract of Chrysophyllum cainito L. in increasing vertebrae trabecular osteoblast cell number in male mice

Ma'arif, Burhan and Aditama, Agnis Pondinekaria (2019) Activity of 96% ethanol extract of Chrysophyllum cainito L. in increasing vertebrae trabecular osteoblast cell number in male mice. Asian Journal of Pharmaceutical and Clinical Research, 12 (1). pp. 286-288. ISSN 0974-2441

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Full text available at: http://doi.org/10.22159/ajpcr.2019.v12i1.28994

Abstract

Objectives: This study was conducted to analyze the anti-osteoporosis effect of 96% ethanol extract from Chrysophyllum cainito L. leaves, which suspected to contain phytoestrogens, in increasing the osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice.

Materials and Methods: The 96% ethanol extract of C. cainito leaves was given to male mice with dose of 2, 4, 8, and 16 mg/20 g BB mice/day after being induced orally with dexamethasone dose of 0.0029 mg/20 g BB mice/day. The positive control used was 0.026 ml/20 g BB mice/day alendronate. After 4 weeks, the increasing of osteoblast cell number in trabecular vertebrae bone of male mice was observed using an optical microscope with 100× zoom in after histomorphometry and hematoxylin-eosin staining methods.

Results: The result showed the significant increasing of osteoblast number in trabecular vertebrae bone of male mice in all groups after being given treatment using 96% ethanol extract of C. cainito, with an effective dose (ED50) value of 9.5 mg/20 g BB mice/day. This increase is suspected due to the phytoestrogens content, which can also act as phytotestosterone in 96% ethanol extract of C. cainito.

Conclusion: This study concluded that 96% ethanol extract of C. cainito has an activity in increasing osteoblast cell number in trabecular vertebra bone of dexamethasone-induced male mice, with an ED50 value of 9.5 mg/20 g BB mice/day.

Item Type: Journal Article
Keywords: Chrysophyllum cainito L.; Phytoestrogens; Osteoporosis
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Faizuddin Harliansyah
Date Deposited: 22 Jan 2019 03:17

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