The influence of elicitor Cu2+ and Fe 2+ to asiaticoside level of pennywort (Centella asiatica L. urban) callus

Savitri, Evika Sandi and Dwi Khoriroh, Feni and Lugiati Sholikhah, Luluk The influence of elicitor Cu2+ and Fe 2+ to asiaticoside level of pennywort (Centella asiatica L. urban) callus. Presented at Malaysia International Biological Symposium 2014, 28th - 29th October 2014, Universiti Putra Malaysia, Putrajaya, Malaysia.

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Abstract

Pennywort (Centella asiatica L. Urban is a medicinal plant used in traditional herbal medicine industry in Indonesia. Production of bioactive compounds through tissue culture can be enhanced by elicitation. This study was an experimental study using completely randomized design.The treatment used is various concentration of metal ions Fe2+ on callus subculture medium namely 0, 90, 100, and 110 mM. with 4 replications to obtain 16 trials. Cu2+ ion concentration treatments used consisted of 4 levels: Cu0 = control (0 mM), Cu1=30 mM , Cu2 = 35 mM, Cu3 =40 mM with 4 replications to obtain 16 trials Elicitor Cu2+ with 40μM concentration resulted in the high levels production of secondary metabolites asiaticoside 4.1595 g / 100g. Elicitor Fe2+ with a concentration of 100 mM asiaticoside produce secondary metabolite 3.836 g / 100g. Elicitor Cu2 + produces higher levels asiaticoside compared with Fe2+.

Item Type: Conference (Paper)
Keywords: elicitor; Centella asiatica L. urban; Cu2+ and Fe 2+; callus
Subjects: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060799 Plant Biology not elsewhere classified
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Sandi Savitri
Date Deposited: 28 May 2019 04:18

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