Isolation and Identification of Chitin and Chitosan Horseshoe Crab Shrimp Shell Using Infrared Spectroscopy

Barroroh, Himmatul and Rifai, D.N. Rizkiyah and Jannah, Akyunul (2019) Isolation and Identification of Chitin and Chitosan Horseshoe Crab Shrimp Shell Using Infrared Spectroscopy. International Journal of Engineering & Technology, 8 (1.9). pp. 372-377. ISSN 2227-524X

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Abstract

Isolation and identification of chitin and chitosan Horseshoe Crab Shrimp Shell has been carried out. There were several reports about chitin isolation on crabs and shrimp shells with different reagent concentrations due to the shell hardness difference. Mechanically, the Horseshoe Crab Shrimp Shell was harder than the two. The characterization of chitin and chitosan is implemented through Infra Red Spectroscopy. Isolation method consists of three steps. First, it was deproteination with various NaOH concentrations of 3.5%, 4.5%, 5.5%, 6.5% and 7.5%. The second was demineralization with various HCl concentrations of 1 M, 1.5 M, 2 M, 2.5 M, and 3 M. The two steps would produce chitin. The third step was deacetylation with a NaOH concentration of 50% to produce chitosan. The results showed that the optimum concentration of NaOH in deproteination step is 4.5% with the optimum concentration of released protein by 601 ppm. In addition, the optimum concentration of HCl in the demineralization step was 2.5 M with remaining ash in the chitin of 0,972%. Moreover, deacetylation degree (D%) of chitin and chitosan were 45.4% and 50.5% respectively. Infrared spectroscopy showed that secondary NH amide stretching of chitin was vanished on chitosan. Meanwhile, secondary C=O amide stretching shifted in chitin and disappeared on chitosan due to deasetylation.

Item Type: Journal Article
Keywords: Horseshoe Crab; mimi; shell; madura; isolation; chitin; chitosan; infrared spectroscopy
Subjects: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules
03 CHEMICAL SCIENCES > 0306 Physical Chemistry (incl. Structural) > 030606 Structural Chemistry and Spectroscopy
Divisions: Faculty of Mathematics and Sciences > Department of Chemistry
Depositing User: Himmatul Barroroh
Date Deposited: 12 Dec 2019 01:10

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