Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice

Tanod, Wendy Alexander and Wahyudi, Didik and Riyadi, Putut Har (2019) Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice. Veterinary World, 12. pp. 1897-1902. ISSN 2231-0916

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Abstract

Background and Aim: The soft coral genus Sarcophyton is a source of cembraneterpen. Sarcophyton is reported to have
anti-inflammatory properties, with the ability to reduce the expression of inducible nitric oxide synthase (iNOS) and inhibit nuclear factor-kappa B (NF-κB) activation. This study aimed to investigate the efficacy of dichloromethane (DCM) extracts
of soft coral Sarcophyton spp. to inhibit the expression of NF-κB and iNOS induced by lipopolysaccharide (LPS).
Materials and Methods: Crude extracts of Sarcophyton spp. were macerated with DCM (1:3 v/v) for 24 h. Thirty-six
Balb/c mice were divided into six treatment groups, namely, normal control (without LPS induction), negative control (LPS induction 4 mg/mL), comparative control (LPS+Dexamethasone 6 mg/kg), and 3 concentration groups extract (LPS+50, 125, and 250 mg/kg). The expression of NF-κB and iNOS was measured in each treatment group.
Results: Flow cytometry analysis showed that the relative number of NF-κB+ cells increased (18.38±1.24%) in LPSinduced mice compared with normal mice (13.24±1.15%). The Sarcophyton spp. DCM extracts decreased the relative
number of NF-κB+ cells (125 mg/kg: 13.96±0.84%). Immunohistochemical analysis with ImmunoMembrane showed that LPS induction in mice increased iNOS expression when compared to normal mice. The Sarcophyton spp. DCM extracts reduced iNOS expression (especially at 125 mg/kg).
Conclusion: DCM extracts of Sarcophyton spp. inhibited the activation of NF-κB, resulting in suppressed iNOS expression,
which directly inhibits NO production.

Item Type: Journal Article
Keywords: Alcyoniidae; anti-inflammatory; lipopolysaccharide; nitric oxide; soft coral
Subjects: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060602 Animal Physiology - Cell
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Didik Wahyudi
Date Deposited: 09 Dec 2019 22:47

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