Genetic variability of porang populations (Amorphophallus muelleri) in West Java and Central Java based on trnL intron sequences

Wahyudi, Didik, Azrianingsih, Rodiyati and Mastuti, Retno (2013) Genetic variability of porang populations (Amorphophallus muelleri) in West Java and Central Java based on trnL intron sequences. Journal of Biodiversity and Environmental Sciences (JBES), 3 (9). pp. 31-41. ISSN 2220-6663

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Abstract

Porang (A. muelleri) is herbaceous plant which is included in the family of Araceae. Previous studies showed morphological and genetic variation in A. muelleri. The suitable molecular marker used to indicate genetic variation is trnL intron. It is based on, trnL intron is non-coding regions of chloroplast genome which evolved more rapidly than coding region. The present study is aimed to find out genetic variability and phylogenetic relationship among A. muelleri populations in Central Java and West Java which is based on trnL intron sequences. Porang samples are obtained from central Java (Karangtengah, Wonogiri, Grobogan and Brebes) and West Java (Cisompet Garut). trnL sequences were analyzed by maximum parsimony and neighbor joining
method. trnL intron has singletone variation as many as 13 sites, i.e on the order of squences: 49, 109, 144, 145, 159, 253, 297, 298, 300, 302, 379, 461, 463. In addition, trnL intron has 9 parsimony informative sites, i.e on the order of squences: 160, 184, 299, 409, 452, 454, 513, 527, 534. The highest number of haplotypes came from Grobogan populations with values diversity of haplotype is 1, while the least number of haplotypes came from Karangtengah and Wonogiri populations with diversity of haplotype is 0.000. phylogenetic tree shows the
clustering of populations into three clade, first clade is A. variabilis and second clade are A. ochroleucus, A.
longituberosus and A. sumawongii as outgroup and thirth clade divided into two subclade porang are clustered
based on their population in Central Java and West Java

Item Type:	Journal Article
Keywords:	Amorphophallus muelleri; trnL intron; genetic variability
Subjects:	06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060302 Biogeography and Phylogeography 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	12 Jun 2020 14:35

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