Isolasi dan uji kompatibilitas bakteri hidrolitik dari tanah tempat pemrosesan akhir Talangagung, Kabupaten Malang

Fitriasari, Prilya Dewi and Amalia, Nanda and Farkhiyah, Susiyanti (2020) Isolasi dan uji kompatibilitas bakteri hidrolitik dari tanah tempat pemrosesan akhir Talangagung, Kabupaten Malang. Berita Biologi, 19 (1). pp. 151-156. ISSN 2337-8751

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Abstract

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.

Item Type: Journal Article
Keywords: bacteria; compatibility; consortium; enzymes
Subjects: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology
06 BIOLOGICAL SCIENCES > 0605 Microbiology
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Prilya Dewi Fitriasari
Date Deposited: 08 Sep 2020 02:16

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