Molecular identification of trichoderma isolates from sugarcane bagasse based on Internal Transcribed Spacer (ITS) rDNA

Rukmana, Siti, Ansori, Arif N. M., Kusala, Muhammad K. J., Utami, Ulfah, Wahyudi, Didik and Mandasari, Andita A. (2020) Molecular identification of trichoderma isolates from sugarcane bagasse based on Internal Transcribed Spacer (ITS) rDNA. Research Journal of Pharmacy and Technology, 13 (7). pp. 3300-3304. ISSN 0974-3618 (Print); 0974-360X (Online)

Text 6807.pdf - Published Version Restricted to Repository staff only Download (206kB)

Abstract

This study aimed to identify Trichoderma from sugarcane bagasse based on iinternal transcribed spacer (ITS) rDNA. The sample used was pure isolate of Trichoderma from sugarcane bagasse. DNA samples were isolated using the modified CTAB method. DNA was amplified using the primers ITS1 and ITS4. Interestingly, the results showed that Trichoderma genomic DNA has a concentration of 92.56 mg with a purity of 1.91. The amplicon of Trichoderma DNA is about 600 bp, whereas phylogenetic analysis shows that the sample of Trichoderma from sugarcane bagasse is one group with Trichoderma harzianum, Trichoderma piluliferum, Trichoderma sp. SQR339, Hypocrea nigricans, and Trichoderma sp. NFML CH12 BB. 15, Trichoderma aureoviride, Hypocrea lixii, and Trichoderma BAB-4585.

Item Type:	Journal Article
Keywords:	Internal transcribed spacer (ITS); rDNA; sugarcane bagasse; trichoderma
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060409 Molecular Evolution
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Ulfah Utami
Date Deposited:	15 Oct 2020 14:03

Downloads

Origin of downloads

Actions (login required)

View Item