The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap

Ma'unatin, Anik, Harijono, Harijono, Zubaidah, Elok and Rifa'i, Muhaimin (2020) The isolation of exopolysaccharide-producing lactic acid bacteria from lontar (Borassus flabellifer L.) sap. Iranian Journal of Microbiology, 12 (5). pp. 437-444. ISSN pISSN: 2008-3289, eISSN: 2008-4447

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Abstract

Background and Objectives: Lontar (Borassus flabellifer L.) is widely grown in Indonesia and one of its products is palm sap. Palm sap contains a high level of sugar, making it suitable as a medium to increase the lactic acid bacteria (LAB) production of exopolysaccharides (EPS). This study aimed to isolate the EPS-producing LAB from palm sap and evaluate its EPS production. LAB isolation was carried out on MRS agar containing 0.5% CaCO3.

Materials and Methods: The screening and production of EPS were carried out on MRS media supplemented with 10%
sucrose. The molecular identification of the selected EPS-producing LAB was based on 16S rDNA. A quantitative analysis of EPS polymer dry mass and total sugar was conducted using one-way ANOVA.

Results: In this study, five EPS-producing LABs were found: Fructobacillus fructosus N4, Leuconostoc mesenteroides N5, Leuconostoc mesenteroides N7, Leuconostoc mesenteroides N9, and Fructobacillus fructosus N10. The highest EPS yield in liquid media was 10.997 ± 1.591 g/L by Leuconostoc mesenteroides N7, whereas the lowest was 4.505 ± 0.459 g/L by Fructobacillus fructosus N10.

Item Type:	Journal Article
Keywords:	Borassus flabellifer; Exopolysaccharide; Fructobacillus fructosus; Lactic acid bacteria; Leuconostoc mesenteroides
Subjects:	03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics
Divisions:	Faculty of Mathematics and Sciences > Department of Chemistry
Depositing User:	Anik Ma'unatin
Date Deposited:	23 Nov 2020 10:03

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