Ma'arif, Burhan, Rosa, Nabila, Dianti, Meilina Ratna, Firdausy, Alif Firman, Laswati, Hening and Agil, Mangestuti (2020) Cytotoxicity test of 96% ethanol extract of Semanggi (Marsilea crenata Presl.) on hFOB 1.19 cells using the Microtetrazolium (MTT) Assay method. FARMASIS: Jurnal Sains Farmasi, 1 (1). pp. 26-32.
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Abstract
Semanggi (Marsilea crenata Presl.) is a plant that has been used by Indonesian people, especially East Java, as a food ingredient for decades. Marsilea crenata Presl. was known to contain phytoestrogens which have structure and function similar to estrogen in the human body. The aim of this study was to do a cytotoxicity test and determine the IC50 value of 96% ethanol extract of Marsilea crenata Presl. against hFOB 1.19 cells. The method used was microtetrazolium (MTT) assay method with microplate reader. The cells were cultured in the microplate-96 well and were given the extract with various doses of 62.5 ppm; 125 ppm; 250 ppm; 500 ppm; 1000 ppm; 2000 ppm and 4000 ppm. Then 100μL of MTT reagent was added, incubated for 2-4 hours and 10% SDS was added as a stopper and readings were carried out using an microplate reader. The results of these readings were used as a calculation of the IC50 value which indicated the cytotoxicity level of 96% ethanol extract of Marsilea crenata Presl. leaves. The IC50 value obtained was 2074.965 μg/ml, and based on these results, the 96% ethanol extract of Marsilea crenata Presl. was concluded to be safe and not cytotoxic for developing into a medicinal product.
| Item Type: | Journal Article |
|---|---|
| Keywords: | Marsilea crenata presl.; cytotoxicity test; 1.19 hFOB cells; MTT Assay |
| Subjects: | 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences |
| Divisions: | Faculty of Medical and Health Sciences > Department of Pharmacy |
| Depositing User: | Burhan Ma'arif |
| Date Deposited: | 25 Nov 2020 15:37 |
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