The expression of drought resistance GmDREB-1 gene on soybean (Glycine max L. Merr) as result of EMS mutation induction (Etyl Methane Sulfonate)

Savitri, Evika Sandi and Resmisari, Ruri Siti (2019) The expression of drought resistance GmDREB-1 gene on soybean (Glycine max L. Merr) as result of EMS mutation induction (Etyl Methane Sulfonate). Presented at International Conference on Science, Engineering, Built Environment, and Social Science, 28 Nov 2016, Bandung, West Java.

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Abstract

Abiotic stresses, particularly drought, are harmful for the plant productivity. Mutagenesis is widely used as method that is potential to increase the variability of crop development. Mutagenic using EMS (Ethyl Methane Sulfonate) shows more effective results than using other mutagens. DREB1 gene is a subclass of DREB gene which is a transcription factor and it also serves as a key regulatory response of plants to drought stress. Mutation induction combines concentration treatment and immersion time in EMS. RNA isolation employs RNeasy Plant Mini Kit (Qiagen). GmDREB-1 expression is analyzed using Real Time PCR (Quantitative Real-time Polymerase Chain Reaction or Q-PCR). The analysis shows that mutation treatment with EMS using 0.07% concentration with a wide range of immersion time indicates that GmDREB-1 expression is higher than other treatments. The results of this study proved that mutation induction using EMS can be used as an alternative to plant breeding to obtain drought resistant soybean. Induction mutation useful for improvement genetic variability of plant.

Item Type:	Conference (Paper)
Keywords:	GmDREB-1, mutation induction; EMS
Subjects:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl. Microarray and other genome-wide approaches) 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics) 10 TECHNOLOGY > 1001 Agricultural Biotechnology > 100101 Agricultural Biotechnology Diagnostics (incl. Biosensors) 10 TECHNOLOGY > 1001 Agricultural Biotechnology > 100106 Genetically Modified Horticulture Plants 10 TECHNOLOGY > 1001 Agricultural Biotechnology
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Sandi Savitri
Date Deposited:	27 Nov 2020 17:45

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