Hayati, Elok Kamilah, Rahmawan, Achmad Zaki Farid, Husna, H., Nihayatul, Ana, Madjid, Armeida Dwi Ridhowati and Mutiah, Roihatul (2019) The separation optimization of berberine in Anting-Anting Plants (Acalypha indica Linn) using High Performance Liquid Chromatography (HPLC). Presented at Annual Conference of Science and Technology, 30 Aug 2018, Malang, East Java, Indonesia.
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Abstract
Anting-Anting plants (Acalypha Indica Linn) is a plant widely found in Indonesia. This plant has potential as an antimalarial, antidiabetic, and antitoxic drug. Ethyl acetate extract of this plant contained isoquinoline alkaloid, berberine which confirmed by LC-MS spectra on m/z 321,2561. Then, thisstudy was conducted to determine the optimum conditions of separation and determination the berberine level existed on ethyl acetate extract of Anting-Anting using HPLC. The separation was optimized by varying flow rate of eluent (0.6 mL/min, 0.8 mL/min, 1.0 mL/min), mobile phase (methanol: TFA 0.1% (50:50 v/v) and acetonitrile: TFA 0.1% (60:40 v/v)). The separation was using HPLC with C18 (ODS) 4.6 x 250 mm, 5 μm column, isocratic method at various wavelengths (290 nm, 315 nm, and 345 nm). The optimum separation condition was obtained at flow rate 1 mL/min with mobile phase methanol: TFA 0.1% (50:50 v/v) and UV detector wavelength at 345 nm. The result showed that the berberine level of Anting-anting in ethyl acetate fraction was 11,82030 µg/mL.
Item Type: | Conference (Paper) |
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Subjects: | 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules 11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified |
Divisions: | Faculty of Medical and Health Sciences > Department of Pharmacy |
Depositing User: | Dr. M.Kes. Roihatul Muti'ah |
Date Deposited: | 27 Nov 2020 20:50 |
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