The separation optimization of berberine in Anting-Anting Plants (Acalypha indica Linn) using High Performance Liquid Chromatography (HPLC)

Hayati, Elok Kamilah and Rahmawan, Achmad Zaki Farid and Husna, H. and Nihayatul, Ana and Madjid, Armeida Dwi Ridhowati and Mutiah, Roihatul (2019) The separation optimization of berberine in Anting-Anting Plants (Acalypha indica Linn) using High Performance Liquid Chromatography (HPLC). Presented at Annual Conference of Science and Technology, 30 Aug 2018, Malang, East Java, Indonesia.

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Abstract

Anting-Anting plants (Acalypha Indica Linn) is a plant widely found in Indonesia. This plant has potential as an antimalarial, antidiabetic, and antitoxic drug. Ethyl acetate extract of this plant contained isoquinoline alkaloid, berberine which confirmed by LC-MS spectra on m/z 321,2561. Then, thisstudy was conducted to determine the optimum conditions of separation and determination the berberine level existed on ethyl acetate extract of Anting-Anting using HPLC. The separation was optimized by varying flow rate of eluent (0.6 mL/min, 0.8 mL/min, 1.0 mL/min), mobile phase (methanol: TFA 0.1% (50:50 v/v) and acetonitrile: TFA 0.1% (60:40 v/v)). The separation was using HPLC with C18 (ODS) 4.6 x 250 mm, 5 μm column, isocratic method at various wavelengths (290 nm, 315 nm, and 345 nm). The optimum separation condition was obtained at flow rate 1 mL/min with mobile phase methanol: TFA 0.1% (50:50 v/v) and UV detector wavelength at 345 nm. The result showed that the berberine level of Anting-anting in ethyl acetate fraction was 11,82030 µg/mL.

Item Type: Conference (Paper)
Subjects: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules
11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Dr. M.Kes. Roihatul Muti'ah
Date Deposited: 27 Nov 2020 20:50

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