Intraspecific variation of thirteen pisang Ambon cultivars (Musa Acuminata CV. AAA) from East Java and Central Java (Indonesia) based on random amplified polymorphic DNA (RAPD) marker

Wahyudi, Didik, Adnin, Nurul Izatul and Hapsari, Lia (2020) Intraspecific variation of thirteen pisang Ambon cultivars (Musa Acuminata CV. AAA) from East Java and Central Java (Indonesia) based on random amplified polymorphic DNA (RAPD) marker. International Journal of Conservation Science, 11 (4). ISSN 2067-533X

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Abstract

Pisang Ambon (M. acuminata cv. AAA) is popular dessert banana in Indonesia, included as Gros Michel.Particularly at Java, there are some variation of Pisang Ambon local cultivars which need to be clearly classified. This study was aims to reveal the intraspecific genetic variation and subgrouping within thirteen of Pisang Ambon cultivars from East Java and Central Java based on RAPD markers. Twenty RAPD markers were performed i.e. OPA1-OPA20. Morphologically, Pisang Ambon cultivars are mostly distinguished by their fruit size, peel colours, pulp colors and pulp taste. Indeed, the fruits are reflecting their distinct morphological or perceptual characteristics. The amplification bands produced in each sample have various lengths, ranging from 140 bp to 1500 bp. In total, there are 101 amplified. In total, 101 characters (RAPD bands) were subjected to dissimilarity distance and hierarchical clustering analysis. The tree resulted from RAPD profiles was clearly illustrated the genetic intraspecific relationship within Pisang Ambon cultivars in Java bands identified. It comprised of 86.82% polymorphic and 13.18% monomorphic bands. The derived cultivars of Pisang Ambon were separated into four sub-clusters, with low dissimilarity distances ranged 0.24 to 0.51. The conclusion of this study revealed that RAPD analysis was effective to study the genetic relationship in intraspecific level of Pisang Ambon cultivars. Furthermore, the finding of this study was showed that within Pisang Ambon cultivars were genetically variable.

Item Type:	Journal Article
Keywords:	Genetic variation; Gros Michel; Musa acuminata; Pisang Ambon; RAPD; Triploid
Subjects:	06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060305 Evolution of Developmental Systems 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060310 Plant Systematics and Taxonomy 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060408 Genomics
Divisions:	Faculty of Mathematics and Sciences > Department of Biology
Depositing User:	Didik Wahyudi
Date Deposited:	30 Nov 2020 21:12

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