The influence of Kesambi leaf extract (Schleichera oleosa) on proliferation granulosa cell goat ovarium (Caprus aegagrus HIRCUS) in vitro

Hanifah, Lil (2020) The influence of Kesambi leaf extract (Schleichera oleosa) on proliferation granulosa cell goat ovarium (Caprus aegagrus HIRCUS) in vitro. Biota, 6 (1). pp. 19-25. ISSN 2528-262X

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Abstract

Kesambi (Schleichera oleosa) belonging to the Sapindaceae family has very important phytochemical elements including terpenoids, falvonoids, phenolic acids, betulins, betulin acids and others, so that they have enormous benefits in the process of antimicrobials, antioxidants, anticancer and can be used for the production of biodiesel. One of the potency of antioxidant ability as an antioxidant is due to its phenolic compounds. The presence of antioxidant activity in cells can affect the defense of cell protein membranes, so that the cell's ability to carry out division is optimal. In vitro this is mostly done by adding hormones to culture cell media or natural compound substances to increase cell proliferation. This study is an experimental study using a Complete Random Design (CRD) with 5 treatments and 4 replications as follows: K0: granulosa cells and culture media without treatment (0 µl), P1: granulosa cells and culture media which were given 1% porch leaf extract (30 µl), P2: granulosa cells and culture media which were given kesambi leaf extract 1.5% (45 µl), P3: granulosa cells and culture media were given 2% (60 µl) kesambi leaf extract and P4: granulosa cells and culture media were given a 2.5% (75 µl) kesambi leaf extract. In this study, there was an influence on the administration of kesambi (Schleichera oleosa) leaf extract to the proliferation of goat ovarian granulosa cells in vitro in the P3 (2%) treatment ie a dose of 60 µl. However, it did not have a significant effect on the viability of goat ovarian granulosa cells.

Item Type: Journal Article
Keywords: kesambi leaf extract; proliferation; granulosa cells
Subjects: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060802 Animal Cell and Molecular Biology
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Lil LH Hanifah
Date Deposited: 07 Dec 2020 14:14

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