Activity and toxicity of Eleutherine palmifolia (L.) Merr. extract on BALB/c mice colitis-associated colon cancer model

Mutiah, Roihatul, Sari, Riza Ambar, Firsyaradha, Wahyi Yucha, Listiyana, Anik, Indrawijaya, Yen Yen Ari, Wafi, Abdul ORCID: https://orcid.org/0000-0002-9717-0094, Suryadinata, Arief, Susilowati, Retno and Rahmawati, Ana (2020) Activity and toxicity of Eleutherine palmifolia (L.) Merr. extract on BALB/c mice colitis-associated colon cancer model. Asian Pacific Journal of Cancer Prevention, 21 (12). pp. 1-8. ISSN 1513-7368

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Abstract

Objective: Eleutherine palmifolia (L.) Merr. extract (EPE) containing isoliquiritigenin and oxyresveratrol is believed to be an anticancer agent. This study evaluates colon histopathology, TNF-α, TGF-β, and hepatotoxicity on BALB/c mice colitis-associated colon cancer (CAC) model treated with EPE. Methods: In vivo study was performed on BALB/c mice CAC model induced by 10 mg/kgBW AOM on the first day followed by administration that each cycle consisted of 5% DSS in water for seven days and regular water for seven days. The indicators of the formation of CAC were observed by a fecal occult blood test (FOBT) and serum amyloid α (SAA) test. The treatment was conducted once a week started from the seventh week up to the twentieth week with six treatment groups: I was administrated by regular water only (negative control), II was administrated by AOM and DSS only (positive control), III was administrated by doxorubicin, IV-VI were treated by EPE (0.25 mg/kg BW, 0.50 mg/kg BW, and 1.00 mg/kg BW) respectively. The colon and liver’s histopathology was observed using hematoxylin-eosin (HE) staining, TNF-α with immunohistochemistry (IHC), and level measurement of TGF-β colon with ELISA reader. The data were used one-way ANOVA followed by post hoc as statistical analysis. Results: The administration of EPE increased the expression of TNF-α, the total of goblet cells of the colon, and decreased the level of TGF-β. Administration of EPE 0.50 mg/20g BW decreased a liver histopathological score but induced a histopathological alteration of the liver at a dose of 1.00 mg/20g BW. Conclusion: This study indicate that EPE could be recommended as a colon anticancer through increase the goblet cells, induce apoptosis through increase TNF-α, and decrease TGF-β.

Item Type:	Journal Article
Keywords:	Eleutherine palmifolia (L.) Merr ; colon cancer TNF-α TGF-β hepatotoxicity
Subjects:	11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111201 Cancer Cell Biology
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Dr. M.Kes. Roihatul Muti'ah
Date Deposited:	16 Mar 2021 13:46

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