The Effect of Calotropis gigantea Leaves Extract on Fibrosarcoma Growth and Caspase 3 Expression

Mutiah, Roihatul and Griana, Tias Pramesti and Ula, Qonitah Nurul and Andhyarto, Yanu (2016) The Effect of Calotropis gigantea Leaves Extract on Fibrosarcoma Growth and Caspase 3 Expression. International Journal of Pharmaceutical and Clinical Research, 8 (3). pp. 167-171. ISSN 0975-1556

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Abstract

The effect of Calotropis gigantea (CG) leaves extract on fibrosarcoma growth and Caspase 3 expression have been evaluated. Mile mice were divided into 5 groups with 7 mice per group, (1) Negative control (CMC-Na 0.5%), (2) Positive control (Methotrexate 2,5 mg/kgBW), (3) 50 mg/Kg BW of CG, (4) 100 mg/Kg BW of CG, (5) 150 mg/Kg BW of CG. Mice body weights were observed before induced by cancer agent. Then, all mice were injected by 0.1 mL DMBA 25 μg/0.1 mL acetone by subcutaneous injection two times a week for 6 weeks. After treatment, all mice were sacrificed by euthanasia method based on Guideline for the Euthanasia of Animals. Then, mice nodules were taken and washed with PBS. Nodules were weighted and kept in the formaldehyde solution. Then immunohistochemical caspase-3 specimens were made. C. gigantea leaves extract at a dose of 100 and 150 mg/Kg BW were able to inhibit the growth of fibrosarcoma in experimental animals induced DMBA. This extract also improved the apoptotic index of cell, respectively for 20.9 %; 21.5 % and 24.6 %, and increased the caspase-3 expression significantly. Based on those data, C. gigantea leaves ethanol extract was potential to develop as anticancer phytopharmaca agent.

Item Type: Journal Article
Keywords: Calotropis gigantea; fibrosarcoma; apoptosis; caspase-3
Subjects: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules
03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030405 Molecular Medicine
11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology
Divisions: Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User: Dr. M.Kes. Roihatul Muti'ah
Date Deposited: 06 Oct 2016 03:25

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