Effect of synbiotics Lactobacillus Casei AP and inulin extract Dahlia Pinnata L. in enteropathogenic Escherichia Coli - induced diarrhea

Kusmiyati, Nur and Sunarti, Sunarti and Wahyuningsih, Tutik Dwi and Widodo, Widodo (2018) Effect of synbiotics Lactobacillus Casei AP and inulin extract Dahlia Pinnata L. in enteropathogenic Escherichia Coli - induced diarrhea. Presented at 2018 1st International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC), Yogyakarta, Indonesia, 23-24 Oktober 2018, Yogyakarta.

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Abstract

The purpose of this study was to evaluate the effect of synbiotic products extract of inulin dahlia tuber and L.casei AP on mice Balb-C strain induced enteropathogen escherichia coli. The steps of this study were the preparation of EPEC bacteria cell suspension and synbiotic products; treatment in an animal, weigh of mice, fecal and pH value; IgA level by ELISA and SCFA analysis by GC and GC-MS. This study shows that synbiotic products can reduce diarrhea in these experimental animals by increasing body weight (24.05 g), feces weight (785.35 mg) and decreased fecal pH (7.08). Total of the LAB in mice's fecal 9.01 (log CFU/g) was higher than total Escherichia coli 7.45 (log CFU/g) after treatment by synbiotic product. IgA levels in the duodenum (1.65 ng/mL) and ileum (1.45 ng/mL) were higher than jejunum (1.36 ng/mL). SCFA analysis with GC and GC-MS showed that the difference in a metabolite of treatment group 2 dose of diarrhea compared to other groups due to butyric acid, isobutene and 2- methyl propionic. The synbiotic effect is stimulating microbial shift, IgA, and production of metabolite optimally at dose 2.

Item Type: Conference (Paper)
Keywords: EPEC; IgA; inulin; L.casei AP; SCFA; synbiotic
Subjects: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Divisions: Faculty of Mathematics and Sciences > Department of Biology
Depositing User: Nur Kusmiyati
Date Deposited: 29 Mar 2021 01:32

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