A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature

Wafi, Abdul, Supriyanto, Ganden and Tjahjandarie, Tjitjik Srie (2016) A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature. Indonesian Journal of Chemistry, 16 (1). pp. 32-35. ISSN 1411-9420

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Abstract

An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO2, bismuth nitrate pentahydrate (Bi(NO3)3.5H2O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 μg/mL and 8-9 min respectively.

Item Type:	Journal Article
Subjects:	03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030101 Analytical Spectrometry 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030107 Sensor Technology (Chemical aspects)
Divisions:	Faculty of Medical and Health Sciences > Department of Pharmacy
Depositing User:	Abdul Wafi
Date Deposited:	01 Nov 2016 13:58

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